동식물위생연구 국제공인연구실 WOAH reference lab 브루셀라병연구실
동식물위생연구에 대한 모든 정보가 있습니다.
WOAH 77차 총회에서 아·태 지역 최초로 브루셀라병 표준실험실로 인정받음(2009년 5월)
이진주 수의연구사
[농림축산검역본부]
실험실-세균질병과
[농림축산검역본부]
Quantitative Rose Bengal Test for diagnosis of bovine brucellosis(2012-01-10) | |||||
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담당부서 | Animal and Plant Health Research | 작성자 | Moon Her | 0 | 2012-01-10 |
Cho Donghee*, Nam Hyangmi, Kim Jongwan, Heo Eunjeong, Cho Yunsang, Hwang Inyeong, Kim Jaemyung, Kim Jongman, Jung Sukchan and More Simon. 2010. Quantitative Rose Bengal Test for diagnosis of bovine brucellosis. J. Immunoassay Immunochem, 31:2, 120 – 130 AbstractThe Rose Bengal Test (RBT) is the most widely used screening test for brucellosis in both humans and animals. Owing to its apparent simplicity of reading, however, interpretations of the RBT results can be affected by personal experience. This study describes a simple way to improve the accuracy and uniformity of reading the RBT reaction by counting the number of agglutinated particles using transparent OHP film with Quantity One, which was originally designed to count the bacterial colony numbers on agar plates. Using this system, the reactivities of three Rose Bengal antigens from different sources against international standard serum (1,000 units, VLA, UK) could be numerically measured: the intensity scale ranged from zero to around 1,600. This system enabled us to compare the antigenicity of Rose Bengal antigens from three different sources by using statistical analyses such as regression and mean intensity. Collectively, mathematical measuring of the reaction intensity used in this study may help interpret subtle test results by providing more reliable data and additional statistical information on the herd. In addition, the method would also be applicable to other agglutination test for other diseases. |
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