동식물위생연구 국제공인연구실 WOAH reference lab 브루셀라병연구실
동식물위생연구에 대한 모든 정보가 있습니다.
WOAH 77차 총회에서 아·태 지역 최초로 브루셀라병 표준실험실로 인정받음(2009년 5월)
이진주 수의연구사
[농림축산검역본부]
실험실-세균질병과
[농림축산검역본부]
The development of a selective medium for the Brucella abortus strains and its comparison with the currently recommended and used medium(2012-01-10) | |||||
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담당부서 | Animal and Plant Health Research | 작성자 | Moon Her | 0 | 2012-01-10 |
Her M*, Cho DH, Kang SI, Cho YS, Hwang IY, Bae YC, Yoon H, Heo YR, Jung SC, Yoo H. 2010. The development of a selective medium for the Brucella abortus strains and its comparison with the currently recommended and used medium. Diagn Microbiol Infect Dis. 67(1):15-21 AbstractThe Brucella spp. are fastidious and relatively slow-growing organisms. The isolation of such strains in a variety of specimens often requires the use of a selective medium to reduce or eliminate the growth of unexpected microorganisms. The modified Brucella selective (MBS) medium, which contains improved antibiotic mixtures, erythritol as the only carbon source, and neutral red as a pH indicator, showed good selectivity for the Brucella abortus strains, including the RB51 vaccine strain. Erythritol in the MBS medium was able to promote and/or recover the delayed growth of the B. abortus strains through the antibiotic mixtures. The Brucella colonies, which assumed a pinkish color at their central part, were easily differentiated from other organisms. The MBS medium also allows the isolation of the Brucella strains even in contaminated specimens and/or in specimens containing small numbers of viable organisms. Moreover, this medium can be applied to environmental samples for the isolation of the Brucella strains, and it can thus offer epidemiologic traceback sources for the dissemination or transfer of diseases. Therefore, the MBS medium can be applied as a useful tool of important control measures in the eradication programs. |
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