Designated as WOAH Reference Laboratory for Brucellosis at 77th WOAH general session in May, 2009.
Dr. Jin-Ju Lee
[Animal and Plant Quarantine Agency]
Bacterial Disease Division
[Animal and Plant Quarantine Agency]
Rapid and specific identification of Brucella abortus using the loop-mediated isothermal amplification (LAMP) assay(2015-04-14) | |||||
---|---|---|---|---|---|
Part | 동식물위생연구부 | Charger | 이진주 | date | 2015-04-14 |
Rapid and specific identification of Brucella abortus using the loop-mediated isothermal amplification (LAMP) assay. Comp Immunol Microbiol Infect Dis. 2015 Mar 14.pii: S0147-9571(15)00014-4. Kang SI, Her M, Kim JY, Lee JJ, Lee K, Sung SR, Jung SC. Abstract A rapid and accurate diagnosis of brucellosis is required to reduce and prevent the spread of disease among animals and the risk of transfer to humans. In this study, a Brucella abortus-specific (Ba) LAMP assay was developed, that had six primers designed from the BruAb2_0168 region of chromosome I. The specificity of this LAMP assay was confirmed with Brucella reference strains, B. abortus vaccine strains, B. abortus isolates and phylogenetically or serologically related strains. The detection limit of target DNA was up to 20fg/μl within 60min. The sensitivity of the new LAMP assay was equal to or slightly higher than other PCR based assays. Moreover, this Ba-LAMP assay could specifically amplify all B. abortus biovars compared to previous PCR assays. To our knowledge, this is the first report of specific detection of B. abortus using a LAMP assay. The Ba-LAMP assay can offer a rapid, sensitive and accurate diagnosis of bovine brucellosis in the field. |
|||||
file | 0files
|
||||
|