Designated as WOAH Reference Laboratory for Brucellosis at 77th WOAH general session in May, 2009.
Dr. Jin-Ju Lee
[Animal and Plant Quarantine Agency]
Bacterial Disease Division
[Animal and Plant Quarantine Agency]
Genetic comparison of B. canis isolates by the MLVA assay in South Korea(2012-01-11) | |||||
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Part | Animal and Plant Health Research | Charger | Moon Her | date | 2012-01-11 |
Sung-Il Kang, Eun Jeong Heo, Donghee Cho, Jong Wan Kim, Ji-Yeon Kim, Suk-Chan Jung, Moon Her*. 2011. Genetic comparison of B. canis isolates by the MLVA assay in South Korea. J. Vet. Med. Sci. 73(6), 779-786 AbstractThe multiple-locus VNTR analysis (MLVA) assay is a method frequently employed as a molecular epidemiological tool for Brucella genetic fingerprinting. The purpose of this study was to assess the genotyping of 77 B. canis isolates from 14 different dog breeding farms in Korea by the MLVA assay and to compare the epidemiological relationships between the Korean isolates and foreign ones. Simpson's diversity index for 17 loci showed a range from 0 to 0.846 in 77 B. canis isolates. B. canis isolates in Korea were observed to have high genetic diversity at the most variable loci and were divided into 30 distinct genotypes by phylogenetic analysis. Some B. canis isolates were closely related to previously typed isolates in other countries. The MLVA assay can be helpful to analyze the epidemiological correlation of B. canis isolates in domestic pet animals and to track the geographic origin by comparing the genetic patterns with foreign isolates. Therefore, the MLVA assay will be useful as a tool for control and preventive measures of canine brucellosis.
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