Molecular Characterization and Genogrouping of an Aquatic Birnavirus GC1 Isolated from Rockfish Sebastes schlegeli in Korea ( 07/06/27 ) | |||||
---|---|---|---|---|---|
Part | Charger | mckim00 | date | 07/06/27 | |
Poster presentation in the Korean Society of Veterinary Science on April 20, 2007.
Results and conclusion
Results:
The nucleotide sequence of VP1 gene of GC1 isolate was found to be 2,776 bp long. The predicted molecular weight
of the VP1 was 94,263 Daltons, and the gene was known to contain a single large open reading frame (ORF) encoding
the 846-amino acids. We also observed that the VP1 sequence contained 6 potential Asn-X-Ser/Thr motifs, 8 potential
Ser phosphorylation sites and 1 Tyr phophorylation site. The amino acid sequence of VP1 did not contain the GDD
motif, which exists commonly in the RdRps of RNA viruses; however, we could identify the Leu-Lys-Asn (LKN) motif at
position 521. On comparing the amino acid sequences of VP1, it was found that GC1 strain shares 97%-98% homology
with MABVs; 94% homology with the IPNV strains of aquabirnaviruses found mainly in the USA, Japan, and Korea;
87%-89% homology with the IPNV strains of aquabirnaviruses from Spain; 46%-47% homology with the avibirnaviruses;
and 29% homology with the entomobirnaviruses. In the phylogenetic cladograms, the genetic relationships among
the 22 birnaviruses were established and the viruses, including GC1, were clustered into 5 genogroups that generally
correlated with the geographic origin of the viruses and the water environment of the host.
Conclusions:
The study of genetic relationships using a phylogenetic cladogram revealed that Korean aquatic birnavirus GC1 is
more closely related to genogroup 1 than genogroup 2. This result indicates that genetic relationships may be influenced by the geographical distributions of the isolates.
|
|||||
file | 1개
(전체저장 버튼 클릭 시 압축파일로 저장됩니다.)
|
||||