FOOT AND MOUTH DISEASE IN THE REPUBLIC OF KOREA
Suspicion

 

(Date of last previously reported outbreak: 1934).

Emergency report

Text of a fax received on 28 March 2000 from Dr Joo-Ho Lee, Director of Animal Health Division, Ministry of Agriculture and Forestry, Seoul:

Report date: 27 March 2000.

Nature of diagnosis: clinical and laboratory.

Date of initial detection of animal health incident: 20 March 2000.

Outbreaks:

Location

No. of outbreaks

Papyung county, Paju city, Kyunggi province, about 5 km from the Demilitarised Zone, in a basin, well isolated by a wall of mountains


1 farm

Description of affected population: dairy farm comprising 12 milking cows and 3 calves.

Total number of animals in the outbreak:

species

susceptible

cases

deaths

destroyed

slaughtered

bov

15

15

0

15

0

Diagnosis: clinical signs observed were: depression, anorexia, excessive salivation, lameness, vesicles and ulcers on feet, mouth, tongue and teats, and a sudden drop in milk production.

- On 20 March 2000, one lactating cow showed clinical signs.

- The disease rapidly spread within the herd to the other cows and the calves (to 10 animals on 22 March and 4 animals on 24 March 2000).

- On 24 March 2000, the owner of the affected herd notified the disease.

- Clinical inspection and laboratory diagnosis by the National Veterinary Research and Quarantine Service were performed on 25 March 2000.

A. Laboratories where diagnosis was made: National Veterinary Research and Quarantine Service (Anyang, Republic of Korea).

B. Diagnostic tests used:

- RT-PCR(1) (3D polymerase, IRES(2) and 1D common regions): positive;

- DNA sequencing (3D polymerase region): partial;

- viral antigen typing by ELISA(3): positive;

- antibody detection using liquid-phase blocking ELISA: positive;

- indirect ELISA using recombinant 3D polymerase: positive;

- transmission electron microscopy: virus particles in vesicular fluid;

- virus isolation: in progress.

C. Causal agent: FMD virus type O is suspected by ELISA for antibody detection and viral antigen typing (using diagnostic reagent and kit made by Pirbright Laboratory, United Kingdom), and by RT-PCR.

Source of agent / origin of infection: under investigation.

Control measures during reporting period: stamping out (26 March 2000); setting up of protection and surveillance zones. Necessary procedures have been implemented.

(1) RT-PCR: reverse transcriptase polymerase chain reaction.

(2) IRES: internal ribosome entry site.

(3) ELISA: enzyme-linked immunosorbent assay.