published paper on Application of a New Portable Microscopic Somatic Cell Counter with Disposable Plastic Chip for Milk Analysis ( 07/08/10 ) | |||||
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Part | Charger | moonjs | date | 07/08/10 | |
In this study, we developed a portable C-reader system (C-reader™) that utilizes the capillary flow of a micro-fluidic chamber by a surface modification of the hydrophilicity. The micro-fluidic technology of disposable microchips allows for low reagent consumption, and a combination of ready-to-use reagents makes the daily work easier. The repeatability test of the C-reader™ using 10 composite bovine milk samples satisfied the recommended values for SCC equipment. In addition, an acceptable accuracy level of the natural logarithmic transformed SCC, SCCt [Ln (SCC/1,000): ± 0.059 to 0.112] was achieved using composite raw milk samples and various somatic cell standard solutions from the American Eastern Laboratory and the Korean National Veterinary Research & Quarantine Service. After testing 875 composite milk samples, the C-reader™ showed a high correlation coefficient (R2, 0,935 to 0.964) and a low mean difference value in SCCt (-0.088 to 0.004) when compared with 3 automatic commercialized somatic cell counters (Fossomatic™ 4000, Somacount® 150, and Somascope™). In conclusion, the C-reader system is a new easy-to-use, automatic on-farm method with acceptable repeatability and accuracy for measuring the SCC in large dairies and smaller laboratories. (Journal of Dairy Science 90:2253-2259)
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