Designated as WOAH Reference Laboratory for Brucellosis at 77th WOAH general session in May, 2009.
Dr. Jin-Ju Lee
[Animal and Plant Quarantine Agency]
Bacterial Disease Division
[Animal and Plant Quarantine Agency]
A new Brucella canis species-specific PCR assay for the diagnosis of canine brucellosis(2015-04-14) | |||||
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Part | 동식물위생연구부 | Charger | 이진주 | date | 2015-04-14 |
A new Brucella canis species-specific PCR assay for the diagnosis of canine brucellosis. Comp Immunol Microbiol Infect Dis. 2014 Sep;37(4):237-41. Kang SI, Lee SE, Kim JY, Lee K, Kim JW, Lee HK, Sung SR, Heo YR, Jung SC, Her M. Abstract Brucellosis is a zoonotic disease that is transmitted from animals to humans, and the development of a rapid, accurate, and widely available identification method is essential for diagnosing this disease. In this study, we developed a new Brucella canis species-specific (BcSS) PCR assay and evaluated its specificity and sensitivity. A specific PCR primer set was designed based on the BCAN_B0548-0549 region in chromosome II of B. canis. The PCR detection for B. canis included amplification of a 300-bp product that is, not found on other Brucella species or, genetically or serologically related bacteria. The detection limit of BcSS-PCR assay was 6pg/μl by DNA dilution, or 3×10(3) colony-forming units (CFU) in the buffy coats separated from whole blood experimentally inoculated with B. canis. Using the buffy coat in this PCR assay resulted in approximately 100-times higher sensitivity for B. canis as compared to detect directly from whole blood. This is the first report of a species-specific PCR assay to detect B. canis, and the new assay will provide a valuable tool for the diagnosis of B. canis infection |
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