published paper on the Antibacterial and Immunostimulative Effect of the Chitosan-oligosaccharides on Murine Infection by Staphylococcus aureus isolated from Mastitis Cow ( 07/08/10 ) | |||||
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Part | Charger | moonjs | date | 07/08/10 | |
Based on our previous study evaluating the in vivo cure efficacy of chitosan on bovine mastitis, more water-soluble OCHT with a high degree of deacetylation and low molecular weight was prepared for obtaining high antibiotic efficacy. Moreover, the in vitro antibacterial activity of the OCHT against Staphylococcus aureus isolated from cow with mastitis as well as its in vivo immunostimulative effect on ICR mice were investigated. Compared with control group, the growth of S. aureus was inhibited significantly in groups treated with OCHT ranging from 0.0001% to 0.5% (P<0.05) within 5 to 10 minutes after treatment. Additionally, the surface of the OCHT-treated bacteria was expanded, distorted, and burst compared with that of the control bacteria as determined by electron microscopic observation. The proportion of monocytes was found to increase significantly (P<0.05), and the levels of IL-6 and IFN-γ sharply increased (P<0.01) at l hr after the peritoneal inoculation of the OCHT (0.5 to 1 mg per mouse). Mice challenged intraperitoneally with S. aureus (2.5×108 CFU) following oral treatment of the OCHT (0.5 to 2mg per day) for 7 days showed a higher survival rate (70-100%) compared with that (10%) of the control. With strong antibacterial activity against S. aureus isolated from mastitis cow as well as the immunomodulatory and protective effects on murine infection by S. aureus, we suggest the OCHT prepared in this study as a potential agent for prevention and treatment of bovine mastitis. (Appl Microbiol Biotechnol 75 989-978)
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